Steptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties.
Physical State: Sterile-filtered liquid
Product is stable for about 6 weeks at 2-8°C as an undiluted liquid -
DO NOT FREEZE. Prepare working dilution fresh each day. For extended
storage, add an equal volume of glycerol ( ACS grade or better) for a
final concentration of 50%, and store at -20°C as a liquid. Note: after
the addition of glycerol, the concentration of protein and buffer salts
is one-half of the original.
Expiration date: one year from the date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
ELISA:- 1-2 µg / ml
Western Blot:- 0.1-1 µg / ml
Histo-/Cyto-Chemistry:- 1-2 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Streptavidin, Phosphatase conjugated
- Product Code: S80016-1mg